(Solution Document) DNA Polymerase can distinguish between dNTPs and rNTPs because of discriminator amino acids in the enzyme's nucleotide-binding pocket.


DNA Polymerase can distinguish between dNTPs and rNTPs because of discriminator amino acids in the enzyme's nucleotide-binding pocket. These amino acids occupy the space where the 2'OH group of an incoming rNTP would need to reside in order to properly position the substrates for catalysis. These discriminator amino acids usually have large R groups, which sterically exclude the ribose 2'OH. If you experimentally mutate/change the discriminator amino acids to glycines, predict the effect that this change might have on DNA polymerase. 

 







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